|
KMID : 0545120170270101877
|
|
Journal of Microbiology and Biotechnology
2017 Volume.27 No. 10 p.1877 ~ p.1884
|
|
|
Lactoferrin Protects Human Mesenchymal Stem Cells from Oxidative Stress-Induced Senescence and Apoptosis
|
|
Park Soon-Yong
Jeong Ae-Jin
Kim Geun-Young
Jo A-Ra
Lee Joo-Eon
Leem Sun-Hee
Yoon Joung-Hahn
Ye Sang-Kyu
Chung Jin-Woong
|
|
|
Abstract
|
|
|
|
Mesenchymal stem cells (MSCs) have been suggested as a primary candidate for cell therapy applications because they have self-renewal and differentiation capabilities. Although they can be expanded in ex vivo system, clinical application of these cells is still limited because they survive poorly and undergo senescence or apoptosis when transplanted and exposed to environmental factors such as oxidative stress. Thus, reducing oxidative stress is expected to improve the efficacy of MSC therapy. The milk protein lactoferrin is a multifunctional ironbinding glycoprotein that plays various roles, including reduction of oxidative stress. Thus, we explored the effect of lactoferrin on oxidative stress-induced senescence and apoptosis of human MSCs (hMSCs). Measurement of reactive oxygen species (ROS) revealed that lactoferrin inhibited the production of hydrogen peroxide-induced intracellular ROS, suggesting lactoferrin as a good candidate as an antioxidant in hMSCs. Pretreatment of lactoferrin suppressed hydrogen peroxide-induced senescence of hMSCs. In addition, lactoferrin reduced hydrogen peroxide-induced apoptosis via inhibition of caspase-3 and Akt activation. These results demonstrate that lactoferrin can be a promising factor to protect hMSCs from oxidative stress-induced senescence and apoptosis, thus increasing the efficacy of MSC therapy.
|
|
|
KEYWORD
|
|
|
Apoptosis, lactoferrin, mesenchymal stem cells, oxidative stress, senescence
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|